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KMID : 1123920040180061805
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Korean Journal of Oriental Physiology and Pathology
2004 Volume.18 No. 6 p.1805 ~ p.1809
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Cytoprotective Effects of Radix Curcumae Aromaticae in Human Umbilical Vein Endothelial Cells
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Seo Eun-A
Chung Hun-Taeg
Ko Kwang-Hak
Kwon Kang-Beom
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Abstract
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In order to validate the use of Radix Curcumae Aromaticae as an anti-inflammatory drug in the traditional Korean medicine, I have investigated the effect of water-soluble extract of Radix Curcumae Aromaticae (ECA) on the expression of inducible heme oxygenase-1 (HO-1), which ha.s anti-inflammatory and cytoprotective effects stimulates, in human umbilical vein endothelial cells (HUVECs) stimulated with a high dose of pro-inflammatory tumor necrosis factor-alpha (TNF-α). The extract protected dose-dependently HUVECs against TNF-α-induced apoptosis, as measured qualitatively by a nuclear staining method using the fluoresoence DAPI and quantitatively by a flow cytometry using fluoresce-enhanced Annexin V antibody, and significantly Increased HO-1 expression, as determined by Western blotting analysis using anti-HO-1 antibody. Biockage of HO-1 activity by a pharmacological inhibitor reversed cytoprotection afforded by the extract, and treatment with carbon monoxide, one of HO-1 metabolites, resulted in cytoprotection comparable to the extract. These results suggest that ECA may have therapeutic potential in the control of endothelial disorders caused by inflammatory cytokines.
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KEYWORD
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Radix Curcumae Aromaticae, cytokines, tumor necrosis factor-alpha Human umbilical vein endothelial cells, Heme Oxygenase-1
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